Olga M. Soboleva, Candidate of biological sciences, associate professor, associate professor of the sub-department of microbiology, immunology and virology, Kemerovo State Medical University (22 A Voroshilova street, Kemerovo, Russia), E-mail: firstname.lastname@example.org
Ekaterina P. Kondratenko, Doctor of agricultural sciences, professor, professor of the sub-department of agronomy, breeding and seed production, Kuzbass State Agricultural Academy (5 Markovtseva street, Kemerovo, Russia), E-mail: email@example.com
Andrey S. Sukhikh, Candidate of pharmaceutical sciences, associate professor, senior researcher, Central Research Laboratory, Kemerovo State Medical University (22 A Voroshilova street, Kemerovo, Russia), E-mail: Suhih_as@list.ru
Marina G. Kurbanova, Doctor of engineering sciences, professor, head of the sub-department of food technology of animal origin, Kemerovo State University (6 Krasnaya street, Kemerovo, Russia), E-mail: firstname.lastname@example.org
Alexander Yu. Prosekov, Doctor of engineering sciences, professor, head of the sub-department of bionanotechnology, Kemerovo State University (6 Krasnaya street, Kemerovo, Russia), E-mail: email@example.com
Background. The obtained data indicate the potential role of fatty acids with very long chain (VLCFA) as signaling molecules in the management of both biotic and abiotic stress, including osmotic stress. The purpose of the research is to study the effect of the electromagnetic microwave field (EMF) and osmotic stress on the change of the RFC profile in the leaves of barley seedlings.
Materials and methods. The research was carried out on monthly barley seedlings grown in soil culture in a mixture of peat and sand under laboratory conditions. The scheme of the experiment included six options: 1) control, without microwave treatment, normal humidification; 2) microwave treatment with a power of 0,42 kW, frequency of 2,45 GHz, with an exposure of 11 seconds, normal humidification; 3) microwave treatment with a power of 0,70 kW, frequency of 2,45 GHz, with an exposure of 11 seconds, normal humidification; 4–6) the same options, but grown in conditions of water scarcity. The content of fatty acids was determined by mass spectrometry.
Results. The effect of electromagnetic fields with a power of 0,42 kW when growing barley seedlings under normal water supply conditions in relation to growing them in conditions of water scarcity was manifested in an increase in the content of erucic acid by 1,6 times, and high power (0,70 kW) – by 1,9 times. The increase in the content of arachidic and begenic fatty acids. It is shown that the combined effect of microwave EMF and water deficiency changes the profile of fatty acids with a very long chain, which is expressed in a significant increase in erucic acid in all experimental variants.
Conclusions. When the plant body is affected by such abiotic factors as drought and an ultra-high frequency electromagnetic field of medium and high power, a change in the profile of fatty acids with a very long chain is observed. Changes in the profile of the VLCFA in leaves under the influence of water stress and EMF are associated with the activation of the main adaptive systems in the body of barley seedlings.
very long chain fatty acids, fatty acid composition, water deficit, seedlings, barley, electromagnetic microwave field, drought
1. Bettaieb I., Zakhama N., Wannes W.A. [et al.]. Water deficit effects on Salvia officinalis fatty acids and essential oils composition. Sci. Hortic. 2009;120:271–275. doi:10.1016/j.scienta.2008.10.016
2. De Bigault Du Granrut A., Cacas J.L. How very-long-chain fatty acids could signal stressful conditions in plants? Frontiers in plant science. 2016;7:1490. doi:10.3389/ fpls.2016.01490
3. Zhu X., Xiong L. Putative megaenzyme DWA1 plays essential roles in drought resistance by regulating stress-induced wax deposition in rice. Proc. Natl. Acad. Sci. U.S.A. 2013;110:17 790–17 795. doi:10.1073/pnas.1316412110
4. Xue D. [et al.]. Molecular and evolutionary mechanisms of cuticular wax for plant drought toleranc. Frontiers in plant science. 2017;8(621):1–12. doi:10.3389/fpls.2017. 00621
5. Zhang J. China’s success in increasing per capita food production. J. Exp. Bot. 2011; 62:3707–3711. doi:10.1093/jxb/err132
6. González A., Ayerbe L. Effect of terminal water stress on leaf epicuticular wax load, residual transpiration and grain yield in barley. Euphytica. 2010;172:341–349. doi:10.1007/s10681-009-0027-0
7. Soboleva O.M., Kondratenko E.P., Sukhikh A.S. Profile of higher fatty acids of barley seeds after processing by electromagnetic waves of the microwave range. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Estestvennye nauki = University proceedings. Volga region. Natural sciences. 2019;4:5–15. doi:10.21685/2307-9150-2019-4-1. (In Russ.)
8. Brown A.P., Slabas A.R., Rafferty J.B. Fatty acid biosynthesis in plants-metabolic pathways, structure and organization. Lipids in photosynthesis. Dordrecht: Springer, 2009:11–34.
9. Zakharova Yu.V. Effect of phospholipases of Candidaalbicans fungi on the cell wall and biological properties of bifidobacteria. Uspekhi meditsinskoy mikologii = Advances in medical mycology. 2018;18:77–81. (In Russ.)
10. Moradbeigi L. [et al.]. Effect of Drought Stress and Delay Cultivation on Grain Yield, Oil Yield and Fatty Acids Composition in Canola. Journal of agricultural science (University of Tabriz). 2019;29(2):135–151.